ed by polyamide on silica adsorbent (PA/SiO2) at room temperature was studied with FTIR Spectroscopy. The obtained IR spectra showed that the amide group CONH- of the PA molecule of PA/SiO2 adsorbed the active group of tea polyphenol molecule through hydrogen bonding and did not adsorb the caffeine molecule. The surface hydroxyl group on the SiO2 of PA/SiO2 was the reactive group which adsorbed caffeine and tea polyphend molecules, and the tea polyphenol and caffeine molecules can also adsorb each othe via hydrogen-bonding. Substances like amino acids were not adsorbed by PA/SiO2 and thus easily separated.
Key words Tea polyphenol, Extraction, IR spectra, Caffeine, Polyamide, Silica
茶叶中含有茶多酚、咖啡因及多种氨基酸等生物活性物质[1],其中茶多酚含量约占茶叶干重的22%.茶多酚是天然抗氧化剂,具有很强的清除自由基作用以及抑制癌变、脂质过氧化、抗衰老、